Composite

Part:BBa_K4398011:Design

Designed by: Reinis Muiznieks   Group: iGEM22_Latvia-Riga   (2022-10-11)


Kanamycin resistance transcriptional unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2434
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2434
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
    Illegal BglII site found at 487
    Illegal XhoI site found at 960
    Illegal XhoI site found at 1044
    Illegal XhoI site found at 1316
    Illegal XhoI site found at 1827
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2434
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2434
    Illegal AgeI site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ATG start codon comes from the fusion sites between the promoter and coding sequence


Source

Parts BBa_K4398000; BBa_K4398001; BBa_K4398006

References